γ 32 p atp (New England Biolabs)

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New England Biolabs γ 32 p atp
γ 32 P Atp, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 11283 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/%CE%B3+32+p+atp/T4+Polynucleotide+Kinase/pmc13181404-113-25-37
Average 99 stars, based on 11283 article reviews

γ 32 p atp - by Bioz Stars, 2026-09

99/100 stars

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Sequencing:

Article Title: Phylogenetic and functional characterization of Asgard primases
Article Snippet: .. A 25-nt single-stranded DNA fragment (D25) of the same sequence as the primer used in the extension assay s was labeled at the 5′ terminus using [γ- 32 P]ATP and T4 polynucleotide kinase (New England Biolabs, USA). ..

Article Title: The Fanconi anemia pathway repairs colibactin-induced DNA interstrand cross-links
Article Snippet: Briefly, samples were replicated as described above, and 2 μL of recovered DNA were incubated with 2 U AflIII and 4 U of either EcoRI or BamHI in 50 mM Tris-HCl (pH 8), 100 mM NaCl, 10 mM MgCl 2 in a total volume of 5 μL at 37 °C for 3 h. Reactions were stopped by adding 5 μL of Invitrogen Gel Loading Buffer II. .. Primers for sequencing ladders were radiolabeled by incubating 200 nM sequencing primer with 200 nM [γ− 32 P]ATP and 20 U T4 polynucleotide kinase (NEB) in 1X T4 PNK reaction buffer (70 mM Tris-HCl, 10 mM MgCl 2 , 5 mM DTT; pH 7.6) in a total volume of 50 μL for 30 min at 37 °C. .. T4 PNK was inactivated by heating at 95 °C for 2 min, and the unincorporated radionucleotides were removed using a Micro Bio-Spin column (Bio-Rad) per the manufacturer’s instructions.

Labeling:

Article Title: Chi hotspot control of RecBCD enzyme requires a RecB tether–RecC groove crosspoint interaction
Article Snippet: .. To make the substrate for RecBCD reactions, the plasmid was linearized with Hin dIII, treated with Quick CIP (New England Biolabs), and 5′-end labeled using [γ- 32 P] ATP (3,000 Ci/mmol; Revitty) and phage T4 polynucleotide kinase (New England Biolabs). .. Unincorporated nucleotides were removed with an SR-200 minicolumn (Cytiva).

Article Title: Phylogenetic and functional characterization of Asgard primases
Article Snippet: The labeled template was prepared by annealing a labeled primer (D25 or R25, ) with M13mp18 ssDNA (New England Biolabs, USA) at a 1:1.25 molar ratio. .. D25 and R25 were labeled at the 5′ terminus using [γ- 32 P]ATP and T4 polynucleotide kinase (New England Biolabs, USA). ..

Plasmid Preparation:

Incubation:

Article Title: Evolved DNAzymes and Stable Activation Chemistry Enable High‐Efficiency DNA Ligation
Article Snippet: .. All S1 DNA substrates were ordered from Integrated DNA Technologies (IDT), with sequences shown in Table , and 5′‐radiolabeled with 32 P as follows: 16 nmol S1 DNA substrate was added to a solution containing ∼5 μCi γ‐ 32 P ATP and 5 units of T4 polynucleotide kinase 3′ phosphatase minus (PNK‐3′P, New England Biolabs) in 10 μl of 1× PNK buffer (70 mM Tris‐HCl pH 7.6, 10 mM MgCl 2 , 5 mM DTT; New England Biolabs) and incubated for 30 min at 37°C. ..

Article Title: JULGI coordinates vascular development and leaf patterning through non-cell-autonomous regulation of miR165/166
Article Snippet: .. RNA probes were labelled with [γ− 32 P]-ATP (10 mCi ml −1 ) by incubation with T4 polynucleotide kinase (New England Biolabs) for 30 min at 37 °C. .. The unlabeled radionucleotides were removed using an Illustra MicroSpin G25 column (Amersham).

Article Title: Evolved DNAzymes and Stable Activation Chemistry Enable High-Efficiency DNA Ligation.
Article Snippet: .. All S1 DNA substrates were ordered from Integrated DNA Technologies (IDT), with sequences shown in Table S2 , and 5 ′ - radiolabeled with 32 P as follows: 16 nmol S1 DNA substrate was added to a solution containing ∼ 5 μCi γ-32 P ATP and 5 units of 8 of 10 T4 polynucleotide kinase 3 ′ phosphatase minus (PNK-3 ′ P, New England Biolabs) in 10 μl of 1 × PNK buffer (70 mM Tris-HCl pH 7.6, 10 mM MgCl2 , 5 mM DTT; New England Biolabs) and incubated for 30 min at 37◦C. ..

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